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DTA‐64 inhibits α‐SMA production in the lung epithelial cells. (A) The α‐SMA, vimentin, slug, snail <t>MMP‐2,</t> MMP‐9 and E‐cadherin were detected by Western blotting. (B) Relative density of the above proteins in the lung homogenates. (C) The production of α‐SMA was measured in the lung section by immunofluorescence assay. CD45.2 ‐ cells were gated for α‐SMA measurement (arrow). (D) Gating strategy of α‐SMA, TGF‐β1, vimentin, E‐cadherin in the lung epithelial cells using CD45.2 ‐ (immune cell marker) and CD31 ‐ (endothelial marker), EpCAM + (epithelial cell adhesion molecule marker) by flow cytometry. (E) Proportions of α‐SMA, TGF‐β1, vimentin and E‐cadherin in the CD45.2 ‐ CD31 ‐ EpCAM + lung epithelial cells were shown. All data were shown as mean ± SD (n = 3). * P < 0.05, ** P < 0.01 vs control group. # P < 0.05, ## P < 0.01 vs OVA group
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DTA‐64 inhibits α‐SMA production in the lung epithelial cells. (A) The α‐SMA, vimentin, slug, snail <t>MMP‐2,</t> MMP‐9 and E‐cadherin were detected by Western blotting. (B) Relative density of the above proteins in the lung homogenates. (C) The production of α‐SMA was measured in the lung section by immunofluorescence assay. CD45.2 ‐ cells were gated for α‐SMA measurement (arrow). (D) Gating strategy of α‐SMA, TGF‐β1, vimentin, E‐cadherin in the lung epithelial cells using CD45.2 ‐ (immune cell marker) and CD31 ‐ (endothelial marker), EpCAM + (epithelial cell adhesion molecule marker) by flow cytometry. (E) Proportions of α‐SMA, TGF‐β1, vimentin and E‐cadherin in the CD45.2 ‐ CD31 ‐ EpCAM + lung epithelial cells were shown. All data were shown as mean ± SD (n = 3). * P < 0.05, ** P < 0.01 vs control group. # P < 0.05, ## P < 0.01 vs OVA group
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Predicted pathway activation among the Ozone and CVD studies. Ingenuity pathway software (IPA) scores each pathway based on p-value (IPA Score=-log[p-value]). An IPA Score of >1.3 represents a statistically significant pathway (p-value <0.05). Percent Activity represents the ratio of differentially expressed genes of molecules (defined as having a fold change of |1.2| and a p-value <0.05). Z-score is the predicted activation value of the pathway by IPA. Studies are ordered with ozone first followed by the CVD studies that are predicted to be most active according to their z-score.
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Predicted pathway activation among the Ozone and CVD studies. Ingenuity pathway software (IPA) scores each pathway based on p-value (IPA Score=-log[p-value]). An IPA Score of >1.3 represents a statistically significant pathway (p-value <0.05). Percent Activity represents the ratio of differentially expressed genes of molecules (defined as having a fold change of |1.2| and a p-value <0.05). Z-score is the predicted activation value of the pathway by IPA. Studies are ordered with ozone first followed by the CVD studies that are predicted to be most active according to their z-score.
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Predicted pathway activation among the Ozone and CVD studies. Ingenuity pathway software (IPA) scores each pathway based on p-value (IPA Score=-log[p-value]). An IPA Score of >1.3 represents a statistically significant pathway (p-value <0.05). Percent Activity represents the ratio of differentially expressed genes of molecules (defined as having a fold change of |1.2| and a p-value <0.05). Z-score is the predicted activation value of the pathway by IPA. Studies are ordered with ozone first followed by the CVD studies that are predicted to be most active according to their z-score.
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Image Search Results


DTA‐64 inhibits α‐SMA production in the lung epithelial cells. (A) The α‐SMA, vimentin, slug, snail MMP‐2, MMP‐9 and E‐cadherin were detected by Western blotting. (B) Relative density of the above proteins in the lung homogenates. (C) The production of α‐SMA was measured in the lung section by immunofluorescence assay. CD45.2 ‐ cells were gated for α‐SMA measurement (arrow). (D) Gating strategy of α‐SMA, TGF‐β1, vimentin, E‐cadherin in the lung epithelial cells using CD45.2 ‐ (immune cell marker) and CD31 ‐ (endothelial marker), EpCAM + (epithelial cell adhesion molecule marker) by flow cytometry. (E) Proportions of α‐SMA, TGF‐β1, vimentin and E‐cadherin in the CD45.2 ‐ CD31 ‐ EpCAM + lung epithelial cells were shown. All data were shown as mean ± SD (n = 3). * P < 0.05, ** P < 0.01 vs control group. # P < 0.05, ## P < 0.01 vs OVA group

Journal: Journal of Cellular and Molecular Medicine

Article Title: DEK‐targeting aptamer DTA‐64 attenuates bronchial EMT‐mediated airway remodelling by suppressing TGF‐β1/Smad, MAPK and PI3K signalling pathway in asthma

doi: 10.1111/jcmm.15942

Figure Lengend Snippet: DTA‐64 inhibits α‐SMA production in the lung epithelial cells. (A) The α‐SMA, vimentin, slug, snail MMP‐2, MMP‐9 and E‐cadherin were detected by Western blotting. (B) Relative density of the above proteins in the lung homogenates. (C) The production of α‐SMA was measured in the lung section by immunofluorescence assay. CD45.2 ‐ cells were gated for α‐SMA measurement (arrow). (D) Gating strategy of α‐SMA, TGF‐β1, vimentin, E‐cadherin in the lung epithelial cells using CD45.2 ‐ (immune cell marker) and CD31 ‐ (endothelial marker), EpCAM + (epithelial cell adhesion molecule marker) by flow cytometry. (E) Proportions of α‐SMA, TGF‐β1, vimentin and E‐cadherin in the CD45.2 ‐ CD31 ‐ EpCAM + lung epithelial cells were shown. All data were shown as mean ± SD (n = 3). * P < 0.05, ** P < 0.01 vs control group. # P < 0.05, ## P < 0.01 vs OVA group

Article Snippet: The primary antibodies of IκBα (#4814), phospho (p)‐IκBα (#2859), p‐ERK (#4370), ERK (#4695), Jun N‐terminal kinases (JNK) (#9253), p‐JNK (#9255), p38 (#8690), p‐p38 (#4511) and GAPDH (#2118) were purchased from CST; those of NF‐κB p65 (#207297), PI3K (#18705), PARP (#74290), p‐AKT (#38449), AKT (#38449), p‐mTOR (#109268), mTOR (#32028), TGF‐β1 (#92486), p‐Smad2/3 (#ab63399), Smad2/3 (ab202445), Smad4 (ab230815), MMP‐2 (#92536), MMP‐9 (#38898), snail + slug (#180714), E‐cadherin (#40772), Vimentin (#8978) and α‐SMA (#7817) were purchased from Abcam.

Techniques: Western Blot, Immunofluorescence, Marker, Flow Cytometry

Predicted pathway activation among the Ozone and CVD studies. Ingenuity pathway software (IPA) scores each pathway based on p-value (IPA Score=-log[p-value]). An IPA Score of >1.3 represents a statistically significant pathway (p-value <0.05). Percent Activity represents the ratio of differentially expressed genes of molecules (defined as having a fold change of |1.2| and a p-value <0.05). Z-score is the predicted activation value of the pathway by IPA. Studies are ordered with ozone first followed by the CVD studies that are predicted to be most active according to their z-score.

Journal: Inhalation toxicology

Article Title: Modeling Vascular Inflammation and Atherogenicity after Inhalation of Ambient Levels of Ozone: Exploratory Lessons from Transcriptomics

doi: 10.1080/08958378.2017.1310333

Figure Lengend Snippet: Predicted pathway activation among the Ozone and CVD studies. Ingenuity pathway software (IPA) scores each pathway based on p-value (IPA Score=-log[p-value]). An IPA Score of >1.3 represents a statistically significant pathway (p-value <0.05). Percent Activity represents the ratio of differentially expressed genes of molecules (defined as having a fold change of |1.2| and a p-value <0.05). Z-score is the predicted activation value of the pathway by IPA. Studies are ordered with ozone first followed by the CVD studies that are predicted to be most active according to their z-score.

Article Snippet: The gelatinase activity of the proteins of two of the DEGs, MMP-9 and MMP-2, was measured in archived BAL and serum samples from subjects in the current ozone study using Novex Zymogram gels with 0.1% gelatin as a substrate (ThermoFisher Scientific, Inc., MA, USA) according to manufacturer instructions along with 1 ng of recombinant MMP-9 or MMP-2 control (R&D System, MN, USA), followed by staining with Coomassie Blue.

Techniques: Activation Assay, Software, Activity Assay